Functional mechanical attributes of natural and synthetic gel-based scaffolds in tissue engineering: strain-stiffening effects on apparent elastic modulus and compressive toughness

The accurate identification and determination of elastic modulus and toughness, as well as other functional mechanical attributes of artificial tissues, are of paramount importance in several fields of tissue science, tissue engineering and technology, since biomechanical and biophysical behavior is strongly linked to biological features of the medical implants and tissue-engineering scaffolds. When soft or ultra-soft materials are investigated, a relevant dispersion of elastic modulus values can be achieved, due to the strain-stiffening effects, inducing a typical non-linear behavior of these materials, as a function of strain-range. In this short communication, the Apparent elastic modulus strain-range dependence is estimated from a segmentation of the strain stiffening curve, and the related compressive toughness is investigated and discussed, based on experimental evidence, for 6 different kinds of gels, used for artificial tissue fabrication; experimental results are compared to mechanical properties of native human tissues.